Practical Biotechnology (Practical Lab)
written report
Besides lectures on biological basic concepts, some specific practices in microbiology and bioprocess engineering, and some particular methods used in enzymology, molecular biology and histology are worthy of being directly experimented by students.
– Basic in microbial culture : medium preparation, sterilization, inoculation
– Kinetic study of microbial cultures : measurement of microbial biomass by different techniques, determination of carbon substrate and principal metabolite(s) concentrations at different sampling times.
Example of alcoholic fermentation and enzyme production by B. circulans.
– Labelling and observation of pro/eukaryotic cells.
– Bacterial production of a recombinant protein.
– Determination of Minimal Inhibitory Concentration (MIC)
– Study of enzymatic reactions.
– Determination of the aeration efficiency of a fermentor.
Regardless of their future professional position, the students must be aware of the constraints related to the manipulation, use and inactivation of living cells.
They must be able to take these constraints into account when managing projects dealing with biological issues.
Teaching language: EN
