Separation Sciences
continuous control (MCQs and participation) and a final written exam with documents
Generalities on chromatographic separation methods: principles (interactions and separations), aims, classifications, thin-film and column formats, instrumental aspects, fields of application
Chromatographic interactions and physico-chemical mechanisms controlling separations: volatility, differential interactions, choice of stationary and mobile phases
Fundamental Physical Constants and optimization parameters: retention constants, selectivity, dispersion, resolution
In-line and coupled chromatographic detection : detector characteristics, main detection modes, application to qualitative and quantitative analysis (calibration methods)
Comparison of liquid and gas chromatography and positioning of chromatographic methods in relation to other separation methods. Opening up towards capillary electrophoresis.
The aim of this course is to introduce 1st year students to analytical chromatographic methods before they start practicing in laboratory classes or internships. At the end of this course the students should have a good overview of the different chromatographic approaches and their fields of application. They should have enough knowledge on technological and methodological elements to implement all types of chromatography and optimize separation performances from an analytical point of view.
Teaching language: FR
Documents:
handouts, self evaluation quiz, simulation software